UCCRC Core Facilities: Frank W. Fitch

Monoclonal Antibody Facility

Scientific Director:  Anne Sperling, PhD

Technical Director:  Carol McShan

Phone: 773-702-8756

Website: http://fitchantibodies.bsd.uchicago.edu

The Frank W. Fitch Monoclonal Antibody Facility provides a wide range of services focusing on the generation and production of monoclonal antibodies.  The overall objective of the Facility is to provide investigators with comprehensive hybridoma and protein production services. 

Services

  • Production of novel monoclonal antibodies, including complete immunization, fusion, and ELISA screening services;
  • Custom and routine culturing of antibody producing cell lines, including thawing of cryopreserved cell lines, subcloning, routine culture, and cryopreservation;
  • Customized ELISA services, including performance of standard assays and development of custom assays;
  • Antibody purification, conjugation, and isotype determination;
  • Ascites fluid production;
  • Large-scale protein production using Hollow Fiber Bioreactor (HFB) technology; and
  • Supply of commonly used antibodies.