UCCRC Core Facilities: Immunohistochemistry



Scientific Director:  Anthony Montag, MD

Technical Director:  Terry Shihong Li, MD

Phone: 773-702-9051

Website:  http://ihccf.uchicago.edu/index.htm

The Immunohistochemistry Facility provides support for basic research in cellular structure/function, as well as translational research in clinical applications and molecular medicine. The Facility aims to provide routine histologic and immunohistochemical services in a timely manner using cutting-edge technology and expertise, evaluate and develop new techniques and stains for development, and educate investigators.   

Services

    • Routine histological services including tissue preparation, processing, sectioning, and H&E staining;
    • Specialized tissue sectioning (preparation of 20 serial sections on a single slide);
    • Immunohistochemical services including enzymatic amplification methods and detection systems using conventional and fluorescence microscopy;
    • Development of staining methods for novel antibodies including the identification of appropriate post-sectioning fixatives, antigen retrieval methods, buffers, enzymatic pretreatment, and antibody titration;
    • Repository of control tissue for investigator use;
    • Development of in situ hybridization assays; and
    • Technical support for protocols on all aspects of tissue antigen detection.